argD

168

acetylornithine transaminase

locus: BSU_11220

Molecular weight: 40.74 kDa

pI: 5.93

Protein length: 385 aa

Gene length: 1155 bp

function: biosynthesis of arginine

product: acetylornithine transaminase

essential: no

ec: 2.6.1.11

synonyms: argD

List of homologs in different organisms, belongs to COG4992 (Galperin et al., 2021)

Gene

Coordinates: 1,198,099 → 1,199,256

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The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + N²-acetyl-L-ornithine --> L-glutamate + N-acetyl-L-glutamate 5-semialdehyde (according to UniProt)

Protein family

• [wiki|Class-III pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[wiki|Cofactors]

• PLP (according to UniProt)

Structure

• [PDB|2EH6] (from Aquifex aeolicus, 45% identity)
• [AF|P36839]

Paralogous protein(s)

• [protein|E5F6DF14A4C01B2C90F70E37908D3697BC1B5FEF|yhxA], [protein|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD], [protein|4E1FD02C6FE4103E3B4942BF5221C062CB8D0B9B|gabT]

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE11220 (Δ[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCTCATGAAACAGCCTCCTT,  downstream forward: _UP4_TGATTTTTTTTCGATATAAA
• BKK11220 (Δ[gene|7FA5502FE82D55EC176F68B74B24293EC9B7D4D7|argD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11220 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_GCTCATGAAACAGCCTCCTT,  downstream forward: _UP4_TGATTTTTTTTCGATATAAA

Expression vectors

• pGP3773: expression of Strep-''argD'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
• pGP3776: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
• pGP3792: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [wiki|pWH844], available in [wiki|Jörg Stülke]'s lab

References

• 6096675,24843172,12107147,1312212,7511775