araN

168

[wiki|ABC transporter] for the uptake of α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (sugar-binding protein)

locus: BSU_28750

Molecular weight: 48.48 kDa

pI: 8.56

Protein length: 433 aa

Gene length: 1302 bp

function: uptake of α-1,5-arabinooligosaccharides

product: α-1,5-arabinooligosaccharide [wiki|ABC transporter ](sugar-binding protein)

essential: no

synonyms: araN, yseC

List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

Gene

Coordinates: 2,941,674 → 2,942,975

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The protein

Protein family

• [wiki|Bacterial solute-binding protein 1 family] (according to UniProt)

Structure

• [PDB|5YSB] (from Listeria innocua, 28% identity) [pubmed|29678880]
• [AF|P94528]

[wiki|Localization]

• cell membrane (via [protein|8AF43CA410F3381209196E1B9A4FE66D78020793|araP]-[protein|AE532A8931D516CC676E765D1D1293E73C335D23|araQ]) [Pubmed|10092453]

Biological materials

Mutant

• MGNA-B004 (araN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1003 NBRP B. subtilis, Japan]
• BKE28750 (Δ[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE28750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGCGTTACCCCTTCCA,  downstream forward: _UP4_TAGAATCCCATTCAAAAAGT
• BKK28750 (Δ[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK28750 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGCGTTACCCCTTCCA,  downstream forward: _UP4_TAGAATCCCATTCAAAAAGT

References

• 36815589,10092453,10417639,9084180,20693325,29678880