AraM

• Description: glycerol-1-phosphate dehydrogenase, L-arabinose operon
  
  

Gene name	araM
Synonyms	yseB
Essential	no
Product	glycerol-1-phosphate dehydrogenase
Function	biosynthesis of phosphoglycerolipids
MW, pI	42 kDa, 5.628
Gene length, protein length	1182 bp, 394 aa
Immediate neighbours	araN, araL
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU28760

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: sn-glycerol-1-phosphate + NAD(P)⁺ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)

• Protein family: glycerol-1-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P94527

• KEGG entry: [3]

• E.C. number: 1.1.1.261

Expression and regulation

• Operon: araA-araB-araD-araL-araM-araN-araP-araQ-abfA

• Sigma factor: SigA

• Regulation: repressed by glucose (CcpA)

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

José Manuel Inácio, Carla Costa, Isabel de Sá-Nogueira
Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 9);2345-2355
[PubMed:12949161] [WorldCat.org] [DOI] (P p)

Isabel S-Nogueira, Teresa V Nogueira, Snia Soares, Hermnia de Lencastre
The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression.
Microbiology (Reading): 1997, 143 ( Pt 3);957-969
[PubMed:9084180] [WorldCat.org] [DOI] (P p)

1. Inácio JM, Costa C, de Sá-Nogueira I. (2003) Distinct molecular mechanisms involved in carbon catabolite repression of the arabinose regulon in Bacillus subtilis. Microbiology. Sep;149(Pt 9): 2345-55. PubMed
2. Sa-Nogueira I.M.G., Nogueira T.V., Soares S., de Lencastre H. (1997) The Bacillus subtilis L-arabinose (ara) operon: nucleotide sequence, genetic organization and expression. Microbiology. Mar;143(Pt 3): 957-69. PubMed
3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed