Apt

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Gene name apt
Synonyms
Essential no
Product adenine phosphoribosyltransferase
Function purine salvage and interconversion
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Purine synthesis, Nucleotides (regulation)
MW, pI 18 kDa, 4.701
Gene length, protein length 510 bp, 170 aa
Immediate neighbours relA, yrvE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Apt context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Apt expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, universally conserved proteins, phosphoproteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27610

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)
  • Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-85 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2DY0 (from Escherichia coli k12, 56% identity, 66% similarity)
  • KEGG entry: [3]

Additional information

universally conserved protein

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol Genet Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

H H Saxild, P Nygaard
Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs.
J Bacteriol: 1987, 169(7);2977-83
[PubMed:3110131] [WorldCat.org] [DOI] (P p)