Difference between revisions of "AprE"
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|style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation | |style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation | ||
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| − | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http:// | + | |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU10300 aprE] |
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/AprE AprE] | |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/AprE AprE] | ||
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfN]]'', ''[[yhfO]]'' | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhfN]]'', ''[[yhfO]]'' | ||
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| − | | | + | |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU10300 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU10300 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU10300 Advanced_DNA] |
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Revision as of 12:29, 13 May 2013
- Description: major extracellular alkaline protease
| Gene name | aprE |
| Synonyms | sprE |
| Essential | no |
| Product | extracellular alkaline serine protease (subtilisin E)) |
| Function | protein degradation |
| Gene expression levels in SubtiExpress: aprE | |
| Interactions involving this protein in SubtInteract: AprE | |
| MW, pI | 39 kDa, 9.342 |
| Gene length, protein length | 1143 bp, 381 aa |
| Immediate neighbours | yhfN, yhfO |
| Sequences | Protein DNA Advanced_DNA |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
utilization of nitrogen sources other than amino acids
This gene is a member of the following regulons
AbrB regulon, ScoC regulon, SinR regulon
The gene
Basic information
- Locus tag: BSU10300
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1 (according to Swiss-Prot)
- Protein family: peptidase S8 family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed
Database entries
- Structure: 1SBC
- UniProt: P04189
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Operon: aprE (according to DBTBS)
- Regulatory mechanism:
- Additional information: the mRNA is extremely stable (more than 25 min) PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
- Additional information: The pAPNC213 vector published by Morimoto et al. (2002) is an insertion plasmid for IPTG inducible constructs that can be integrated into the chromosomal aprE locus by replacing the aprE open reading frame via double homologous recombination. PubMed
Labs working on this gene/protein
Your additional remarks
References
Reviews
Original publications
