aprE
168
major extracellular alkaline protease
locus
BSU_10300
Molecular weight
39.37 kDa
pI
9.34
function
protein degradation
product
extracellular alkaline serine protease (subtilisin E)
essential
no
synonyms
aprE, sprE
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
1,104,423 1,105,568
The protein
Catalyzed reaction/ biological activity
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides(according to UniProt)
Protein family
[wiki|peptidase S8 family] (according to UniProt)
[wiki|Domains]
Inhibitor I9 domain (aa 38-109) (according to UniProt)
[wiki|Peptidase S8 domain] (aa 111-380) (according to UniProt)
Structure
[PDB|1SBC]
[AF|P04189]
[wiki|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]
Expression and Regulation
additional information
expression is increased in a [gene|E0BB77C8F1220E931D16A1FA6B75AE8907C76FDB|hag] mutant due to the presence of increased levels of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|degU]-P [pubmed|28800172]
the mRNA is very stable (> 15 min) [pubmed|12884008]
Biological materials
Mutant
KO7 (''nprE aprE epr mpr nprB vpr bpr''), available as BGSC 1A1133
BKE10300 ([gene|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG
BKK10300 ([gene|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK10300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTCTTTACCCTCTCCTTT, downstream forward: _UP4_TAATAGTAAAAAGAAGCAGG
Antibody
**
References
Reviews
Original Publications
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Time of last update: 2024-04-24 10:52:52
Author of last update: Jstuelk