AnsZ

• Description: asparaginase
  
  

Gene name	ansZ
Synonyms	yccC
Essential	no
Product	asparaginase
Function	asparagine utilization
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	39 kDa, 6.15
Gene length, protein length	1125 bp, 375 aa
Immediate neighbours	lmrA, lip
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU02690

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-asparagine + H₂O = L-aspartate + NH₃ (according to Swiss-Prot)

• Protein family: asparaginase 1 family (according to Swiss-Prot)

• Paralogous protein(s): AnsA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1ZCF (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)

• UniProt: O34482

• KEGG entry: [3]

• E.C. number: 3.5.1.1

Expression and regulation

• Operon: ansZ PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: