Difference between revisions of "AnsZ"

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Revision as of 13:41, 18 April 2012

  • Description: asparaginase

Gene name ansZ
Synonyms yccC
Essential no
Product asparaginase
Function asparagine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 39 kDa, 6.15
Gene length, protein length 1125 bp, 375 aa
Immediate neighbours lmrA, lip
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YccC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsZ expression.png




























Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

CodY regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU02690

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
  • Protein family: asparaginase 1 family (according to Swiss-Prot)
  • Paralogous protein(s): AnsA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1ZCF (from Pectobacterium atrosepticum scri1043, 55% identity, 73% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)