Difference between revisions of "AnsB"

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(Biological materials)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:''' GP1153 (del erm) available in the [[Stülke]] lab
+
* '''Mutant:''' GP1153 (del ''ansAB''::''erm'') available in the [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 16:01, 7 February 2011

  • Description: L-aspartase

Gene name ansB
Synonyms
Essential no
Product L-aspartase
Function aspartate degradation
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 52 kDa, 5.69
Gene length, protein length 1425 bp, 475 aa
Immediate neighbours mleN, ansA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AnsB context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

  • Locus tag: BSU23570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate = fumarate + NH3 (according to Swiss-Prot)
  • Protein family: Aspartase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): CitG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 1JSW (from Escherichia coli b, 44% identity, 63% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1153 (del ansAB::erm) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666] [WorldCat.org] [DOI] (I e)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)

D X Sun, P Setlow
Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
J Bacteriol: 1991, 173(12);3831-45
[PubMed:1711029] [WorldCat.org] [DOI] (P p)