Difference between revisions of "AnsB"
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=== Database entries === | === Database entries === | ||
| − | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1JSW 1JSW] (from ''Escherichia coli b'', 44% identity, 63% similarity) {{PubMed|9230045}} |
* '''UniProt:''' [http://www.uniprot.org/uniprot/P26899 P26899] | * '''UniProt:''' [http://www.uniprot.org/uniprot/P26899 P26899] | ||
Revision as of 10:32, 19 February 2010
- Description: L-aspartase
| Gene name | ansB |
| Synonyms | |
| Essential | no |
| Product | L-aspartase |
| Function | aspartate degradation |
| Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn | |
| MW, pI | 52 kDa, 5.69 |
| Gene length, protein length | 1425 bp, 475 aa |
| Immediate neighbours | mleN, ansA |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU23570
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: L-aspartate = fumarate + NH3 (according to Swiss-Prot)
- Protein family: Aspartase subfamily (according to Swiss-Prot)
- Paralogous protein(s): CitG
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- UniProt: P26899
- KEGG entry: [3]
- E.C. number: 4.3.1.1
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346]
[WorldCat.org]
[DOI]
(P p)
D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318]
[WorldCat.org]
[DOI]
(P p)
D X Sun, P Setlow
Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
J Bacteriol: 1991, 173(12);3831-45
[PubMed:1711029]
[WorldCat.org]
[DOI]
(P p)
