AnsA

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  • Description: L-asparaginase

Gene name ansA
Synonyms
Essential no
Product L-asparaginase
Function asparagine degradation
Metabolic function and regulation of this protein in SubtiPathways:
Asp, Asn
MW, pI 36 kDa, 4.455
Gene length, protein length 987 bp, 329 aa
Immediate neighbours ansB, ansR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AnsA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

  • Locus tag: BSU23580

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-asparagine + H2O = L-aspartate + NH3 (according to Swiss-Prot)
  • Protein family: asparaginase 1 family (according to Swiss-Prot)
  • Paralogous protein(s): AnsZ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1153 (del ansAB::erm) available in the Stülke lab
  • Expression vector:
  • lacZ fusion: pGP872 (in pAC5), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)

D X Sun, P Setlow
Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase.
J Bacteriol: 1991, 173(12);3831-45
[PubMed:1711029] [WorldCat.org] [DOI] (P p)