ansA

168

L-asparaginase

locus: BSU_23580

Molecular weight: 36.30 kDa

pI: 4.45

Protein length: 329 aa

Gene length: 990 bp

function: asparagine degradation

product: L-asparaginase

essential: no

ec: 3.5.1.1

synonyms: ansA

List of homologs in different organisms, belongs to COG0252 (Galperin et al., 2021)

Gene

Coordinates: 2,455,819 → 2,456,808

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The protein

Catalyzed reaction/ biological activity

• H₂O + L-asparagine --> L-aspartate + NH₄⁺ (according to UniProt)

Protein family

• asparaginase 1 family (with [protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ], according to UniProt)

[wiki|Domains]

• Asparaginase/glutaminase domain (aa 2-322) (according to UniProt)

Structure

• [PDB|5OT0] (from Thermococcus kodakarensis, 43% identity) [pubmed|29095161]
• [AF|P26900]

Paralogous protein(s)

• [protein|B5301B346FBAAE2D7C37D561FE1DCFDDFAC90D40|ansZ]

Biological materials

Mutant

• GP1153 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''ermC'') available in [wiki|Jörg Stülke]'s lab
• GP4256 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]-[gene|5FAE80F22AEBAE63FC3C872EC651166A65C34DC2|ansB]::''cat'') available in [wiki|Jörg Stülke]'s lab
• BKE23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC,  downstream forward: _UP4_TAAAATGTAAAAACGATAAC
• BKK23580 (Δ[gene|FED8E46AA1C4EFD27796D5DFBBB1663EBF336BFD|ansA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23580 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATACCATGCACCTCTTCAC,  downstream forward: _UP4_TAAAATGTAAAAACGATAAC

lacZ fusion

• pGP872 (in [wiki|pAC5]), available in [wiki|Stülke] lab

References

• 8478318,1711029,11914346,22383849,29095161,33782055,34293360,38483038