Difference between revisions of "AmyE"

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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:'''
+
* '''Locus tag:''' BSU03040
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P00691 P00691]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P00691 P00691]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03040 BSU03040]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU03040]
  
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.1 3.2.1.1]
 
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.2.1.1 3.2.1.1]

Revision as of 09:13, 3 June 2009

  • Description: alpha-amylase

Gene name amyE
Synonyms amyA
Essential no
Product alpha-amylase)
Function starch degradation
MW, pI 72 kDa, 5.85
Gene length, protein length 1980 bp, 660 aa
Immediate neighbours ycgB, ldh
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AmyE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU03040

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 13 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

  • Structure: 1BAG (complex with maltopentaose)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation: repressed by glucose (CcpA)
  • Regulatory mechanism: CcpA: transcription repression
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
  1. Henkin, T. M., Grundy, F. J., Nicholson, W. L. and Chambliss, G. H. (1991) Catabolite repression of -amylase gene expression in Bacillus subtilis involves a trans-acting gene product homologous to the Escherichia coli lacI and galR repressors. Mol. Microbiol. 5, 575-584. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed