alsT

168

putative glutamine transporter

locus: BSU_18120

Molecular weight: 50.14 kDa

pI: 9.39

Protein length: 465 aa

Gene length: 1398 bp

function: uptake of glutamine

product: putative glutamine transporter

essential: no

synonyms: alsT

List of homologs in different organisms, belongs to COG1115 (Galperin et al., 2021)

Gene

Coordinates: 1,938,925 → 1,940,322

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The protein

Protein family

• [wiki|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)

Structure

• [PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 36% identity) [pubmed|30659158]
• [AF|Q45068]

Paralogous protein(s)

• [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|glnT], [protein|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD], [protein|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|yflA]

[wiki|Localization]

• cell membrane (according to Swiss-Prot),  membrane [Pubmed|18763711]

Biological materials

Mutant

• GP1888 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::tet), available in [wiki|Jörg Stülke]'s lab [pubmed|32743959]
• BKE18120 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE18120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC,  downstream forward: _UP4_TAATATAAATATAAAACAAA
• BKK18120 (Δ[gene|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|alsT]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK18120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTTACCTCCCGTTTTC,  downstream forward: _UP4_TAATATAAATATAAAACAAA

References

• 12823818,25755103,18763711,29326168,28835263,31997474,30659158