alsR

168

transcriptional activator of the [gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD] operon

locus: BSU_36020

Molecular weight: 34.18 kDa

pI: 7.29

Protein length: 302 aa

Gene length: 909 bp

function: regulation of acetoin synthesis ([gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD])

product: transcriptional activator ([wiki|LysR family])

essential: no

synonyms: alsR

List of homologs in different organisms, belongs to COG0583 (Galperin et al., 2021)

Gene

Coordinates: 3,711,498  3,712,406

Phenotypes of a mutant

• reduced acetoin production [pubmed|31113899]
• impaired biofilm development [pubmed|31113899]

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The protein

Catalyzed reaction/ biological activity

• binds the promoter region of the [gene|AAA45830B9C5428CADFA5551D33B513E9B1B7C8E|alsS]-[gene|355E70F55A28C6C2C24E8E326EFB673AC303FBEE|alsD] operon in the presence of acetate, activates transcription [pubmed|30039521]

Protein family

• [wiki|LysR family] (according to UniProt)

[wiki|Domains]

• [wiki|HTH lysR-type domain] (aa 1-58) (according to UniProt)

Structure

• [PDB|2H98] (from Acinetobacter baylyi, 34% identity)
• [AF|Q04778]

Effectors of protein activity

• acetate (2 molecules per molecule of AlsR) acts as co-factor that triggers activity [pubmed|30039521]

Biological materials

Mutant

• AMBs3 (spc), available in [wiki|Jörg Stülke]'s lab
• 1A978 ( ''alsR''::''cat''), [Pubmed|7685336], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A978&Search=1A978 BGSC]
• BKE36020 ([gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE36020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAATATGCATTCCTTT,  downstream forward: _UP4_TGAACAATACTTGCAATCTC
• BKK36020 ([gene|8BA7714236EBFDBB9987F1DACC9775AD974C743E|alsR]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK36020 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCAATATGCATTCCTTT,  downstream forward: _UP4_TGAACAATACTTGCAATCTC

labs

• [wiki|Elisabeth Härtig], Braunschweig, Germany [http://www.tu-braunschweig.de/ifm/abt/jahn/groups/bs Homepage]

References

Reviews

• 23046954

Original Publications

• 7685336,21821766,22344650,23576037,22178965,23695583,23836140,30039521,31113899