Difference between revisions of "AlsR"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36020 alsR]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU36020 alsR]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=alsR alsR]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 7.288   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34 kDa, 7.288   

Revision as of 11:49, 7 January 2014

  • Description: transcriptional activator of the alsS-alsD operon

Gene name alsR
Synonyms
Essential no
Product transcriptional activator(LysR family)
Function regulation of acetoin synthesis (alsS-alsD)
Gene expression levels in SubtiExpress: alsR
Metabolic function and regulation of this protein in SubtiPathways:
alsR
MW, pI 34 kDa, 7.288
Gene length, protein length 906 bp, 302 aa
Immediate neighbours alsS, ywrK
Sequences Protein DNA DNA_with_flanks
Genetic context
AlsR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AlsR expression.png















Categories containing this gene/protein

carbon core metabolism, transcription factors and their control

This gene is a member of the following regulons

The AlsR regulon:

The gene

Basic information

  • Locus tag: BSU36020

Phenotypes of a mutant

no acetoin production

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed
  • Inactivation of alsR, sigD and sigW increases competitive fitness of Bacillus subtilis under non-sporulating conditions PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds the promoter region of the alsS-alsD operon in the presence of acetate, activates transcription
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
    • K(D) value for the binding site in the alsS promoter region: 33.5 nM PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: AMBs3 (spc), available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Elisabeth Härtig, Braunschweig, Germany Homepage

Your additional remarks

References

Reviews

Elisabeth Härtig, Dieter Jahn
Regulation of the anaerobic metabolism in Bacillus subtilis.
Adv Microb Physiol: 2012, 61;195-216
[PubMed:23046954] [WorldCat.org] [DOI] (I p)

Original publications

Xian Zhang, Rongzhen Zhang, Teng Bao, Taowei Yang, Meijuan Xu, Huazhong Li, Zhenghong Xu, Zhiming Rao
Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis.
J Ind Microbiol Biotechnol: 2013, 40(9);1067-76
[PubMed:23836140] [WorldCat.org] [DOI] (I p)

Claudia Frädrich, Joern Krausze, Nick Quade, Dirk Heinz, Dieter Jahn, Elisabeth Härtig
Purification, crystallization and preliminary X-ray analysis of the effector domain of AlsR, an LysR-type transcriptional regulator from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2013, 69(Pt 5);581-4
[PubMed:23695583] [WorldCat.org] [DOI] (I p)

Rafael R de Oliveira, Wayne L Nicholson
The LysR-type transcriptional regulator (LTTR) AlsR indirectly regulates expression of the Bacillus subtilis bdhA gene encoding 2,3-butanediol dehydrogenase.
Appl Microbiol Biotechnol: 2013, 97(16);7307-16
[PubMed:23576037] [WorldCat.org] [DOI] (I p)

Wayne L Nicholson
Increased competitive fitness of Bacillus subtilis under nonsporulating conditions via inactivation of pleiotropic regulators AlsR, SigD, and SigW.
Appl Environ Microbiol: 2012, 78(9);3500-3
[PubMed:22344650] [WorldCat.org] [DOI] (I p)

Claudia Frädrich, Anika March, Kerstin Fiege, Anja Hartmann, Dieter Jahn, Elisabeth Härtig
The transcription factor AlsR binds and regulates the promoter of the alsSD operon responsible for acetoin formation in Bacillus subtilis.
J Bacteriol: 2012, 194(5);1100-12
[PubMed:22178965] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl Environ Microbiol: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

M C Renna, N Najimudin, L R Winik, S A Zahler
Regulation of the Bacillus subtilis alsS, alsD, and alsR genes involved in post-exponential-phase production of acetoin.
J Bacteriol: 1993, 175(12);3863-75
[PubMed:7685336] [WorldCat.org] [DOI] (P p)