alr

alr
168

alanine racemase

locus
BSU_04640
Molecular weight
43.10 kDa
pI
8.43
Protein length
Gene length
function
peptidoglycan biosynthesis
product
alanine racemase
essential
yes
ec
5.1.1.1
synonyms
alr, dal, alrA

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0787 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
517,372 → 518,541
Phenotypes of a mutant
reported to be essential [Pubmed|12682299], however deletion mutants have been reported by Ferrari et al. (1985) and Xia et al (2007)  [Pubmed|17602222]
auxotrophy for D-alanine
a [gene|219586A3F378DC38EA076FD39B99D41136BDE722|alaP] [gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr] double mutant is not viable due to the inability to acquire D-alanine [pubmed|33155333]
the mutant is not viable in liquid culture but it can live in a mixed biofilm (with wild type) due to D-ala sharing [pubmed|33594973]
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The protein
Catalyzed reaction/ biological activity
L-alanine --> D-alanine (according to UniProt)
Protein family
alanine racemase family (with [protein|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD], according to UniProt)
Structure
[PDB|1L6G] (with N-[5�-phosphopyridoxyl]-D-alanine, Geobacillus stearothermophilus),  [PDB|1SFT] (Geobacillus stearothermophilus)
[AF|P10725]
Paralogous protein(s)
[protein|E267F2B6448E380B354AE091734E4AC4DB5825EF|yncD]
Expression and Regulation
Operons
genes
[gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]-[gene|052BD59112DBE2F1DAC9DA5A7B0EA8DAF8D6FC47|ndoAI]-[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]
description
[pubmed|22383849]
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[gene|CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344|alr]→[gene|2E5A557E417CB355331CC0555D46A79793EB90B3|ndoA]

2024-03-23 02:50:30

Jstuelk

45

91b7fa89916159e2bc7894e263572f1eeb4a5554

F1ED0F7D4D8E54352AA236431B9C138B1FC5ADBA

Biological materials
Mutant
[http://www.ncbi.nlm.nih.gov/nuccore/402479429?report=genbank&from=517383&to=518551 alr-1], two-base mutation 526AG>T (=R176X), creating a frameshift and an immediate stop codon, available in [http://bgsc.org/ BGSC] 1A4, a D-alanine auxotroph
References
Ferrari E, Henner DJ, Yang MY. Isolation of an Alanine Racemase Gene from Bacillus subtilis and its Use for Plasmid Maintenance in B. subtilis, Nature Biotechnol 1985.Nov;3:1003-7.
3138014,17602222,33594973,33155333

CB0B6C69CB5CFF6FE30C3F95991F309C8DE1E344

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Time of last update: 2024-03-28 12:10:03

Author of last update: Jstuelk