Difference between revisions of "AhrC"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''ahrC''-''[[recN]]''
+
* '''Operon:''' ''[[ahrC]]-[[recN]]'' {{PubMed|2106508}}
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
  
* '''Regulation:''' by small RNA [[sr1]]
+
* '''Regulation:'''  
 +
** by [[sRNA]] [[sr1]]
  
* '''Regulatory mechanism:''' inhibtion of translation upon binding of [[sr1]] to the ''ahrC'' mRNA [http://www.ncbi.nlm.nih.gov/sites/entrez/17020585 PubMed]
+
* '''Regulatory mechanism:'''  
 +
** inhibtion of translation upon binding of [[sr1]] to the ''ahrC'' mRNA [http://www.ncbi.nlm.nih.gov/sites/entrez/17020585 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>17020585 17576690 18455186 18007040 18007039 11856827 11305941 9383188 7565595 1312212, </pubmed>
+
<pubmed>17020585 17576690 18455186 18007040 18007039 11856827 11305941 9383188 7565595 1312212, 2106508</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 19:56, 20 November 2009

  • Description: AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism.

Gene name ahrC
Synonyms argR
Essential no
Product transcriptional regulator
Function transcriptional regulator of arginine metabolic genes
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 16,7 kDa, 5.52
Gene length, protein length 447 bp, 149 amino acids
Immediate neighbours yqxC, recN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AhrC context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU24250

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcriptional activator/ repressor of genes involved in arginine metabolism
  • Protein family: argR family (according to Swiss-Prot)
  • Paralogous protein(s):

Genes controlled by AhrC

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): L-arginine is the co-factor required for transcription repression/ activation
  • Effectors of protein activity:
  • Interactions:
  • Localization: Cytoplasm

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism:
    • inhibtion of translation upon binding of sr1 to the ahrC mRNA PubMed
  • Additional information:

Biological materials

  • Mutant: GP729 (aphA3), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Simon Phillips, Leeds University, UK Homepage

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks

References

James A Garnett, Ferenc Marincs, Simon Baumberg, Peter G Stockley, Simon E V Phillips
Structure and function of the arginine repressor-operator complex from Bacillus subtilis.
J Mol Biol: 2008, 379(2);284-98
[PubMed:18455186] [WorldCat.org] [DOI] (I p)

James A Garnett, Simon Baumberg, Peter G Stockley, Simon E V Phillips
Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2007, 63(Pt 11);918-21
[PubMed:18007040] [WorldCat.org] [DOI] (I p)

James A Garnett, Simon Baumberg, Peter G Stockley, Simon E V Phillips
A high-resolution structure of the DNA-binding domain of AhrC, the arginine repressor/activator protein from Bacillus subtilis.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2007, 63(Pt 11);914-7
[PubMed:18007039] [WorldCat.org] [DOI] (I p)

Nadja Heidrich, Isabella Moll, Sabine Brantl
In vitro analysis of the interaction between the small RNA SR1 and its primary target ahrC mRNA.
Nucleic Acids Res: 2007, 35(13);4331-46
[PubMed:17576690] [WorldCat.org] [DOI] (I p)

Nadja Heidrich, Alberto Chinali, Ulf Gerth, Sabine Brantl
The small untranslated RNA SR1 from the Bacillus subtilis genome is involved in the regulation of arginine catabolism.
Mol Microbiol: 2006, 62(2);520-36
[PubMed:17020585] [WorldCat.org] [DOI] (P p)

Caitríona A Dennis C, Nicholas M Glykos, Mark R Parsons, Simon E V Phillips
The structure of AhrC, the arginine repressor/activator protein from Bacillus subtilis.
Acta Crystallogr D Biol Crystallogr: 2002, 58(Pt 3);421-30
[PubMed:11856827] [WorldCat.org] [DOI] (P p)

K S Makarova, A A Mironov, M S Gelfand
Conservation of the binding site for the arginine repressor in all bacterial lineages.
Genome Biol: 2001, 2(4);RESEARCH0013
[PubMed:11305941] [WorldCat.org] [DOI] (I p)

C M Miller, S Baumberg, P G Stockley
Operator interactions by the Bacillus subtilis arginine repressor/activator, AhrC: novel positioning and DNA-mediated assembly of a transcriptional activator at catabolic sites.
Mol Microbiol: 1997, 26(1);37-48
[PubMed:9383188] [WorldCat.org] [DOI] (P p)

U Klingel, C M Miller, A K North, P G Stockley, S Baumberg
A binding site for activation by the Bacillus subtilis AhrC protein, a repressor/activator of arginine metabolism.
Mol Gen Genet: 1995, 248(3);329-40
[PubMed:7565595] [WorldCat.org] [DOI] (P p)

L G Czaplewski, A K North, M C Smith, S Baumberg, P G Stockley
Purification and initial characterization of AhrC: the regulator of arginine metabolism genes in Bacillus subtilis.
Mol Microbiol: 1992, 6(2);267-75
[PubMed:1312212] [WorldCat.org] [DOI] (P p)

B E Van Hoy, J A Hoch
Characterization of the spoIVB and recN loci of Bacillus subtilis.
J Bacteriol: 1990, 172(3);1306-11
[PubMed:2106508] [WorldCat.org] [DOI] (P p)