AhpF

• Description: alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase
  
  

Gene name	ahpF
Synonyms	ndh
Essential	no
Product	alkyl hydroperoxide reductase (large subunit) / NADH dehydrogenase
Function	resistance against peroxide stres
Gene expression levels in SubtiExpress: ahpF
Metabolic function and regulation of this protein in SubtiPathways: ahpF
MW, pI	54 kDa, 4.705
Gene length, protein length	1527 bp, 509 aa
Immediate neighbours	ahpC, bglA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

resistance against oxidative and electrophile stress, membrane proteins, phosphoproteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU40100

Database entries

• BsubCyc: BSU40100

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NADH + acceptor = NAD⁺ + reduced acceptor (according to Swiss-Prot)

• Protein family: class-II pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylation on (Ser-48 OR Ser-49) PubMed
  • phosphorylated on Arg-457 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU40100

• Structure: 1HYU (from Salmonella typhimurium, 52% identity, 69% similarity) PubMed

• UniProt: P42974

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ahpC-ahpF PubMed

• Expression browser: ahpF PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by H2O2 (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 5837 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 4363 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5017 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: