Difference between revisions of "Adk"

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|style="background:#ABCDEF;" align="center"|'''Function''' || ADP formation  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || ADP formation  
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU01370 adk]
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU01370 adk]
 
|-
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)], [http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[secY]]'', ''[[map]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[secY]]'', ''[[map]]''
 
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11913&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU01370 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU01370 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU01370 Advanced_DNA]
 
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|colspan="2" | '''Genetic context''' <br/> [[Image:adk_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:adk_context.gif]]

Revision as of 11:59, 13 May 2013

  • Description: adenylate kinase

Gene name adk
Synonyms
Essential yes PubMed
Product adenylate kinase
Function ADP formation
Gene expression levels in SubtiExpress: adk
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation), Protein secretion
MW, pI 23 kDa, 4.449
Gene length, protein length 651 bp, 217 aa
Immediate neighbours secY, map
Sequences Protein DNA Advanced_DNA
Genetic context
Adk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Adk expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01370

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + AMP = 2 ADP (according to Swiss-Prot)
  • Protein family: adenylate kinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Thomas H Segall-Shapiro, Peter Q Nguyen, Edgardo D Dos Santos, Saurav Subedi, Justin Judd, Junghae Suh, Jonathan J Silberg
Mesophilic and hyperthermophilic adenylate kinases differ in their tolerance to random fragmentation.
J Mol Biol: 2011, 406(1);135-48
[PubMed:21145325] [WorldCat.org] [DOI] (I p)

Rafael Couñago, Corey J Wilson, Matthew I Peña, Pernilla Wittung-Stafshede, Yousif Shamoo
An adaptive mutation in adenylate kinase that increases organismal fitness is linked to stability-activity trade-offs.
Protein Eng Des Sel: 2008, 21(1);19-27
[PubMed:18093993] [WorldCat.org] [DOI] (P p)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)