Difference between revisions of "AddB"

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(Database entries)
Line 78: Line 78:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Y26 1Y26] (the [[riboswitch]] in complex with adenine)
  
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P23477 P23477]
 
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P23477 P23477]

Revision as of 20:23, 5 June 2009

  • Description: ATP-dependent deoxyribonuclease (subunit B)

Gene name addB
Synonyms
Essential no
Product ATP-dependent deoxyribonuclease (subunit B))
Function DNA repair and recombination
MW, pI 134 kDa, 5.39
Gene length, protein length 3498 bp, 1166 aa
Immediate neighbours yhjR, addA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
AddB context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU10620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1106 (addAB, spc), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Joseph T P Yeeles, Richard Cammack, Mark S Dillingham
An iron-sulfur cluster is essential for the binding of broken DNA by AddAB-type helicase-nucleases.
J Biol Chem: 2009, 284(12);7746-55
[PubMed:19129187] [WorldCat.org] [DOI] (P p)

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed