acsA

168

acetyl-CoA synthetase

locus: BSU_29680

Molecular weight: 64.72 kDa

pI: 5.55

Protein length: 572 aa

Gene length: 1719 bp

function: utilization of acetate, fatty acids

product: acetyl-CoA synthetase)

essential: no

ec: 6.2.1.1

synonyms: acsA

List of homologs in different organisms, belongs to COG0365 (Galperin et al., 2021)

Gene

Coordinates: 3,038,213  3,039,931

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The protein

Catalyzed reaction/ biological activity

• acetate + ATP + CoA --> acetyl-CoA + AMP + diphosphate (according to UniProt)

Protein family

• [wiki|ATP-dependent AMP-binding enzyme family] (according to UniProt)

Structure

• [PDB|2P2F] (from ''Salmonella enterica'', 37% identity) [Pubmed|17497934]
• [AF|P39062]

Modification

• acetylated on Lys-549 by [protein|17C3F56CE859BCD7A282C144C46CC92D3506ABF6|acuA], this results in inactivation  [Pubmed|18487328], deacetylated by [protein|A0A6CB19191A251BB5600C18E039978A9A34933C|srtN] and [protein|1255813797A83D835E0656A2AAAD361B5BB2094B|acuC] deacetylates (and thereby activates) [protein|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]  [Pubmed|19136592]

Paralogous protein(s)

• [protein|48538F67259B25B64A7BC4263E944C7074E8E1B3|ytcI], (35,1%)

Biological materials

Mutant

• GP1212 (''acsA''::''kan''), available in [wiki|Jörg Stülke]'s lab
• BKE29680 ([gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCCAAACATCCCCCTT,  downstream forward: _UP4_ACAATGGAGGATTAAAAGGC
• BKK29680 ([gene|6671E7D85E99D349679F0E9D825DC035D10FFD2E|acsA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29680 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGCCCAAACATCCCCCTT,  downstream forward: _UP4_ACAATGGAGGATTAAAAGGC

References

Reviews

• 15316652,31091420

Original Publications

• 7934817,7913927,18083814,17303131,12618455,19136592,18487328,12850135,16855235,22900538,17497934,29093482