acoA

168

acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)

locus: BSU_08060

Molecular weight: 35.91 kDa

pI: 4.91

Protein length: 333 aa

Gene length: 1002 bp

function: acetoin utilization

product: acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)

essential: no

synonyms: acoA, yfjK

List of homologs in different organisms, belongs to COG1071 (Galperin et al., 2021)

Gene

Coordinates: 879,002 → 880,003

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The protein

[wiki|Cofactors]

• TPP [pubmed|35988322]

Structure

• [PDB|6CFO] (human PDH, E1, 38% identity) [pubmed|29970614]
• [AF|O31404]

Paralogous protein(s)

• [protein|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA], [protein|9F298088C0A9EB7FE140C935AFC9243C6D4DE8AE|bkdAA]

[wiki|Localization]

• cytoplasm (homogeneous) [Pubmed|16479537]

Expression and Regulation

additional information

• the mRNA is very stable (> 15 min) [pubmed|12884008]

Biological materials

Mutant

• MGNA-C280 (acoA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2278 NBRP B. subtilis, Japan]
• BKE08060 (Δ[gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTGCGCCTCCTTCT,  downstream forward: _UP4_TATGAAAAAGGAGGAATGTA
• BKK08060 (Δ[gene|B0A333BDFAE816B48B856A1DD91B73C41584A8E4|acoA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08060 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTGTGCGCCTCCTTCT,  downstream forward: _UP4_TATGAAAAAGGAGGAATGTA

References

Reviews

• 35988322

Original Publications

• 22900538,21815947,11274109,10368162,10666464,12884008,16479537,11274109,16428414,29970614,31113899