Difference between revisions of "Abn2"

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|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yxiA''
+
|''abn2''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yxiA ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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=== Extended information on the protein ===
 
=== Extended information on the protein ===
  
* '''Kinetic information:'''
+
* '''Kinetic information:''' With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
  
 
* '''Domains:'''  
 
* '''Domains:'''  
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* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:'''
+
* '''Localization:''' extracellular [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===
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* '''KEGG entry:'''
 
* '''KEGG entry:'''
  
* '''E.C. number:'''
+
* '''E.C. number:''' 3.2.1.99
  
 
=== Additional information===
 
=== Additional information===
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:'''  
+
* '''Operon:''' ''abn2'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
  
* '''Sigma factor:'''  
+
* '''Sigma factor:''' [[SigA]], [[SigH]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:''' expression is stimulated by arabinose and pectin and repressed by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' unknown [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Isabel de Sa-Nogueira]]
  
 
=Your additional remarks=
 
=Your additional remarks=
 +
  
 
=References=
 
=References=
  
 +
# Inácio JM, and de Sá-Nogueira I(2008) Characterization of ''abn2'' (''yxiA''), encoding a ''Bacillus subtilis'' GH43 arabinanase, Abn2, and its role in arabino-polysaccharide degradation.''J. Bacteriol.'' '''190:''' 4272-4280. [http://www.ncbi.nlm.nih.gov/sites/entrez/18408032 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 13:04, 18 March 2009

  • Description: write here

Gene name abn2
Synonyms yxiA
Essential no
Product
Function unknown
MW, pI 52 kDa, 7.371
Gene length, protein length 1407 bp, 469 aa
Immediate neighbours yxiB, hutP
Gene sequence (+200bp) Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context
YxiA context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information: With linear-alpha-1,5-l-arabinan as the preferred substrate, the enzyme exhibited an apparent K(m) of 2.0 mg ml(-1) and V(max) of 0.25 mmol min(-1) mg(-1) at pH 7.0 and 50°C. PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: extracellular PubMed

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number: 3.2.1.99

Additional information

Expression and regulation

  • Regulation: expression is stimulated by arabinose and pectin and repressed by glucose PubMed
  • Regulatory mechanism: unknown PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabel de Sa-Nogueira

Your additional remarks

References

  1. Inácio JM, and de Sá-Nogueira I(2008) Characterization of abn2 (yxiA), encoding a Bacillus subtilis GH43 arabinanase, Abn2, and its role in arabino-polysaccharide degradation.J. Bacteriol. 190: 4272-4280. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed