Aag

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  • Description: general stress protein, similar to DNA-3-methyladenine glycosidase II

Gene name aag
Synonyms yxlJ
Essential no
Product unknown
Function survival of salt and ethanol stresses
Gene expression levels in SubtiExpress: aag
MW, pI 21 kDa, 6.117
Gene length, protein length 588 bp, 196 aa
Immediate neighbours yxzF, katX
Sequences Protein DNA DNA_with_flanks
Genetic context
YxlJ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Aag expression.png















Categories containing this gene/protein

DNA repair/ recombination, general stress proteins (controlled by SigB)

This gene is a member of the following regulons

SigB regulon

The gene

Basic information

  • Locus tag: BSU38620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed
  • Protein family: DNA glycosylase MPG family (according to Swiss-Prot) AAG family of 3-methyladenine DNA glycosylases
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

Randi M Aamodt, Pål Ø Falnes, Rune F Johansen, Erling Seeberg, Magnar Bjørås
The Bacillus subtilis counterpart of the mammalian 3-methyladenine DNA glycosylase has hypoxanthine and 1,N6-ethenoadenine as preferred substrates.
J Biol Chem: 2004, 279(14);13601-6
[PubMed:14729667] [WorldCat.org] [DOI] (P p)