SigL
- Description: Sigma subunit of the RNA polymerase, Sigma-54, Sigma L
Gene name | sigL |
Synonyms | |
Essential | no |
Product | RNA polymerase sigma-54 factor (sigma-L) |
Function | utilization of arginin, acetoin and fructose, required for cold adaptation |
MW, pI | 49,5 kDa, 7.79 |
Gene length, protein length | 1308 bp, 436 amino acids |
Immediate neighbours | yvfG, yvfH |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU34200
Phenotypes of a mutant
The mutant is cold-sensitive and unable to use arginine as a single carbon source. PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Binding to promoters of the -12, -24 type
- Protein family: sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
- Paralogous protein(s):
Genes/ operons controlled by SigL
acoA-acoB-acoC-acoL, rocA-rocB-rocC, rocD-rocE-rocF, rocG, levD-levE-levF-levG-sacC, ptb-bcd-buk-lpdV-bkdAA-bkdAB-bkdB
Extended information on the protein
- Kinetic information:
- Domains:
- DNA binding domain (H-T-H motif) (324–343)
- pron box domain (413–421)
- 3 x Compositional bias domain (6–21),(32–53),(112–136)
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P24219
- KEGG entry: [3]
Additional information
Expression and regulation
- Operon:
- Regulatory mechanism: CcpA: transcription repression, transcriptional roadblock
- Additional information:
Biological materials
- Mutant: GP146 (aphA3), available in Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- Antibody:
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
Your additional remarks
References
Frank Wiegeshoff, Carsten L Beckering, Michel Debarbouille, Mohamed A Marahiel
Sigma L is important for cold shock adaptation of Bacillus subtilis.
J Bacteriol: 2006, 188(8);3130-3
[PubMed:16585774]
[WorldCat.org]
[DOI]
(P p)
N O Ali, J Bignon, G Rapoport, M Debarbouille
Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis.
J Bacteriol: 2001, 183(8);2497-504
[PubMed:11274109]
[WorldCat.org]
[DOI]
(P p)