FliJ

• Description: flagellar protein required for formation of basal body
  
  

Gene name	fliJ
Synonyms	cheF
Essential	no
Product	flagellar protein required for formation of basal body
Function	movement and chemotaxis
MW, pI	17 kDa, 8.409
Gene length, protein length	441 bp, 147 aa
Immediate neighbours	fliI, ylxF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU16250

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: fliJ family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P20487

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

C W Ying, F Scoffone, A M Albertini, A Galizzi, G W Ordal
Properties of the Bacillus subtilis chemotaxis protein CheF, a homolog of the Salmonella typhimurium flagellar protein FliJ.
J Bacteriol: 1991, 173(11);3584-6
[PubMed:1904439] [WorldCat.org] [DOI] (P p)

C W Ying, G W Ordal
Nucleotide sequence and expression of cheF, an essential gene for chemotaxis in Bacillus subtilis.
J Bacteriol: 1989, 171(3);1631-7
[PubMed:2493450] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed