RadA

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  • Description: 6-O-methylguanine-DNA methyltransferase, negative effector of DisA activity, suppressor of recU and recB mutations, participates in the stabilization and or processing of holliday junction intermediates

Gene name radA
Synonyms sms, yacJ
Essential no
Product 6-O-methylguanine-DNA methyltransferase
Function homologous recombination , control of c-di-AMP formation
Gene expression levels in SubtiExpress: radA
Interactions involving this protein in SubtInteract: RadA
Metabolic function and regulation of this protein in SubtiPathways:
radA
MW, pI 49 kDa, 8.128
Gene length, protein length 1374 bp, 458 aa
Immediate neighbours clpC, disA
Sequences Protein DNA DNA_with_flanks
Genetic context
RadA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RadA expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides, DNA repair/ recombination, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon

The gene

Basic information

  • Locus tag: BSU00870

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: RadA subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism: CtsR: transcription repression PubMed
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Deani L Cooper, Daniel C Boyle, Susan T Lovett
Genetic analysis of Escherichia coli RadA: functional motifs and genetic interactions.
Mol Microbiol: 2015, 95(5);769-79
[PubMed:25484163] [WorldCat.org] [DOI] (I p)

Lei Zhang, Zheng-Guo He
Radiation-sensitive gene A (RadA) targets DisA, DNA integrity scanning protein A, to negatively affect cyclic Di-AMP synthesis activity in Mycobacterium smegmatis.
J Biol Chem: 2013, 288(31);22426-36
[PubMed:23760274] [WorldCat.org] [DOI] (I p)

Begoña Carrasco, M Castillo Cozar, Rudi Lurz, Juan C Alonso, Silvia Ayora
Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation.
J Bacteriol: 2004, 186(17);5557-66
[PubMed:15317759] [WorldCat.org] [DOI] (P p)

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

I Derré, G Rapoport, T Msadek
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.
Mol Microbiol: 1999, 31(1);117-31
[PubMed:9987115] [WorldCat.org] [DOI] (P p)

E Krüger, T Msadek, M Hecker
Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon.
Mol Microbiol: 1996, 20(4);713-23
[PubMed:8793870] [WorldCat.org] [DOI] (P p)