AlkA

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  • Description: DNA-3-methyladenine glycosylase

Gene name alkA
Synonyms
Essential no
Product DNA-3-methyladenine glycosylase
Function adaptive response to alkylative DNA damage
Gene expression levels in SubtiExpress: alkA
MW, pI 35 kDa, 8.147
Gene length, protein length 909 bp, 303 aa
Immediate neighbours glmS, adaA
Sequences Protein DNA DNA_with_flanks
Genetic context
AlkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AlkA expression.png















Categories containing this gene/protein

DNA repair/ recombination, prophage 1, sporulation proteins

This gene is a member of the following regulons

AdaA regulon

The gene

Basic information

  • Locus tag: BSU01800

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine (according to Swiss-Prot)
  • Protein family: alkylbase DNA glycosidase alkA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism: AdaA: positive regulation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

Original publications

F Morohoshi, K Hayashi, N Munkata
Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.
J Bacteriol: 1993, 175(18);6010-7
[PubMed:8376346] [WorldCat.org] [DOI] (P p)