SerA

• Description: phosphoglycerate dehydrogenase
  
  

Gene name	serA
Synonyms
Essential	no
Product	phosphoglycerate dehydrogenase
Function	biosynthesis of serine
Gene expression levels in SubtiExpress: serA
Metabolic function and regulation of this protein in SubtiPathways: serA
MW, pI	56 kDa, 5.617
Gene length, protein length	1575 bp, 525 aa
Immediate neighbours	ypzE, aroC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, membrane proteins, most abundant proteins

The gene

Basic information

• Locus tag: BSU23070

Database entries

• BsubCyc: BSU23070

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 3-phospho-D-glycerate + NAD⁺ = 3-phosphonooxypyruvate + NADH (according to Swiss-Prot)

• Protein family: D-isomer specific 2-hydroxyacid dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • S-cysteinylation after diamide stress (C410)PubMed
  • active site Cys410 is S-bacillithiolated by NaOCl stress in B. subtilis and other Bacillus species PubMed PubMed

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization: membrane PubMed

Database entries

• BsubCyc: BSU23070

• Structure: 1YBA (from E. coli, 30% identity, 52% similarity) PubMed

• UniProt: P35136

• KEGG entry: [3]

• E.C. number: 1.1.1.95

Expression and regulation

• Operon: serA PubMed

• Expression browser: serA PubMed

• Sigma factor:

• Regulation:
  • strongly repressed in response to glucose starvation in M9 medium PubMed

• Regulatory mechanism:

• Additional information:
  • belongs to the 100 most abundant proteins PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 6336 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 12316 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 9076 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 4149 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 5497 PubMed

Biological materials

• Mutant:
  • 1A614 ( serA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion: pGP187 (in pAC7), available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody: