ClpP
Revision as of 14:04, 17 April 2014 by 134.76.70.252 (talk)
- Description: ATP-dependent Clp protease proteolytic subunit (class III heat-shock protein)
Gene name | clpP |
Synonyms | yvdN |
Essential | no |
Product | ATP-dependent Clp protease proteolytic subunit |
Function | protein degradation |
Gene expression levels in SubtiExpress: clpP | |
Interactions involving this protein in SubtInteract: ClpP | |
Metabolic function and regulation of this protein in SubtiPathways: clpP | |
MW, pI | 21 kDa, 5.008 |
Gene length, protein length | 591 bp, 197 aa |
Immediate neighbours | trnQ-Arg, pgcM |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
proteolysis, general stress proteins (controlled by SigB), heat shock proteins, phosphoproteins, most abundant proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU34540
Phenotypes of a mutant
- increased thermotolerance due to increased stabiliy of Spx and thus increased expression of trxA PubMed
Database entries
- BsubCyc: BSU34540
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Hydrolysis of proteins to small peptides in the presence of ATP and magnesium (according to Swiss-Prot) endopeptidase/proteolysis
- Protein family: peptidase S14 family (according to Swiss-Prot) ClpP (IPR001907) InterPro, (PF00574) PFAM
- Paralogous protein(s):
Targets of ClpC-ClpP-dependent protein degradation
Targets of ClpX-ClpP-dependent protein degradation
Extended information on the protein
- Kinetic information:
- Modification:
- phosphorylated on Arg-13 PubMed
- Effectors of protein activity:
Database entries
- BsubCyc: BSU34540
- UniProt: P80244
- KEGG entry: [3]
- E.C. number: 3.4.21.92
Additional information
Expression and regulation
- Operon: clpP PubMed
- Additional information:
- belongs to the 100 most abundant proteins PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium): 4011 PubMed
- number of protein molecules per cell (complex medium with amino acids, without glucose): 11118 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1536 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 731 PubMed
- number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1056 PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion: C-terminal GFP fusions (both single copy and as 2th copy in amyE locus, also as CFP and YFP variants) available in the Leendert Hamoen lab
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Leendert Hamoen, Newcastle University, UK homepage
Your additional remarks
References
Reviews
Original Publications