CheA

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  • Description: two-component sensor kinase, chemotactic signal modulator

Gene name cheA
Synonyms cheN
Essential no
Product two-component sensor kinase
Function chemotactic signal modulator
Gene expression levels in SubtiExpress: cheA
Interactions involving this protein in SubtInteract: CheA
MW, pI 74 kDa, 4.452
Gene length, protein length 2013 bp, 671 aa
Immediate neighbours cheB, cheW
Sequences Protein DNA DNA_with_flanks
Genetic context
CheA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CheA expression.png















Categories containing this gene/protein

protein modification, transcription factors and their control, motility and chemotaxis, membrane proteins, phosphoproteins

This gene is a member of the following regulons

CodY regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: autophosphorylation, phosphorylation of CheY and CheB PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • autophosphorylation on a His residue
  • Cofactor(s):
  • Effectors of protein activity:
    • phosphorylation of CheA is stimulated by interaction with the complex of deaminated McpC-CheD PubMed
    • the kinase activity of CheA is activated by chemoreceptors bound to CheD PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:
    • in minimal medium, CheA is present with 2,600 +/- 560 molecules per cell PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 705 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1580 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 483 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 188 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 62 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Gerald L Hazelbauer, Wing-Cheung Lai
Bacterial chemoreceptors: providing enhanced features to two-component signaling.
Curr Opin Microbiol: 2010, 13(2);124-32
[PubMed:20122866] [WorldCat.org] [DOI] (I p)

Original publications