AsnS

• Description: asparagyl-tRNA synthetase
  
  

Gene name	asnS
Synonyms
Essential	yes PubMed
Product	asparagyl-tRNA synthetase)
Function	translation
Gene expression levels in SubtiExpress: asnS
Metabolic function and regulation of this protein in SubtiPathways: asnS
MW, pI	48 kDa, 5.123
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	dnaD, aspB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes

The gene

Basic information

• Locus tag: BSU22360

Phenotypes of a mutant

essential PubMed

Database entries

• BsubCyc: BSU22360

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn) (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU22360

• Structure: 1X56 (from Pyrococcus horikoshii, 42% identity) PubMed

• UniProt: P39772

• KEGG entry: [2]

• E.C. number: 6.1.1.22

Expression and regulation

• Operon:

• Expression browser: asnS PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 1447 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 5590 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1342 PubMed

• • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1529 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Wataru Iwasaki, Shun-ichi Sekine, Chizu Kuroishi, Seiki Kuramitsu, Mikako Shirouzu, Shigeyuki Yokoyama
Structural basis of the water-assisted asparagine recognition by asparaginyl-tRNA synthetase.
J Mol Biol: 2006, 360(2);329-42
[PubMed:16753178] [WorldCat.org] [DOI] (P p)