AsnS

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  • Description: asparagyl-tRNA synthetase

Gene name asnS
Synonyms
Essential yes PubMed
Product asparagyl-tRNA synthetase)
Function translation
Gene expression levels in SubtiExpress: asnS
Metabolic function and regulation of this protein in SubtiPathways:
asnS
MW, pI 48 kDa, 5.123
Gene length, protein length 1290 bp, 430 aa
Immediate neighbours dnaD, aspB
Sequences Protein DNA DNA_with_flanks
Genetic context
AsnS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AsnS expression.png















Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU22360

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn) (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1X56 (from Pyrococcus horikoshii, 42% identity) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1447 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 5590 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1342 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1529 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References