CdaA

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  • Description: diadenylate cyclase, synthesis of c-di-AMP in vegetative cells

Gene name cdaA
Synonyms ybbP, ybbQ
Essential no
Product diadenylate cyclase
Function synthesis of c-di-AMP
Gene expression levels in SubtiExpress: cdaA
Interactions involving this protein in SubtInteract: CdaA
Metabolic function and regulation of this protein in SubtiPathways:
cdaA
MW, pI 30 kDa, 8.074
Gene length, protein length 819 bp, 273 aa
Immediate neighbours rsiW, cdaR
Sequences Protein DNA DNA_with_flanks
Genetic context
YbbP context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YbbP expression.png















Categories containing this gene/protein

membrane proteins, metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU01750

Phenotypes of a mutant

  • inactivation of cdaA results in severe beta-lactam sensitivity PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • synthesis of c-di-AMP from two molecules of ATP PubMed
  • Protein family:
  • Paralogous protein(s): CdaS

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
    • the interaction with CdaR stimulates the diadenylate cyclase activity of CdaA PubMed

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: the cdaA-cdaR part of the mRNA is very stable (half-life > 15 min) PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 60 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 239 PubMed

Biological materials

  • Expression vector:
    • expression of native cdaA in B. subtilis: pGP1960 (in pBQ200), available in Jörg Stülke's lab
    • expression of cdaA-Strep in B. subtilis suitable for SPINE: pGP1986 (in pGP382), available in Jörg Stülke's lab
    • IPTG inducible expression of cdaA-Strep in E. coli: pGP2564 (in pGP574), available in Jörg Stülke's lab
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab. Respective plasmid: pGP1990.
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ute Römling
Great times for small molecules: c-di-AMP, a second messenger candidate in Bacteria and Archaea.
Sci Signal: 2008, 1(33);pe39
[PubMed:18714086] [WorldCat.org] [DOI] (I e)

Original publications

Felix M P Mehne, Katrin Gunka, Hinnerk Eilers, Christina Herzberg, Volkhard Kaever, Jörg Stülke
Cyclic di-AMP homeostasis in bacillus subtilis: both lack and high level accumulation of the nucleotide are detrimental for cell growth.
J Biol Chem: 2013, 288(3);2004-17
[PubMed:23192352] [WorldCat.org] [DOI] (I p)

Yun Luo, John D Helmann
Analysis of the role of Bacillus subtilis σ(M) in β-lactam resistance reveals an essential role for c-di-AMP in peptidoglycan homeostasis.
Mol Microbiol: 2012, 83(3);623-39
[PubMed:22211522] [WorldCat.org] [DOI] (I p)

Yaara Oppenheimer-Shaanan, Ezequiel Wexselblatt, Jehoshua Katzhendler, Eylon Yavin, Sigal Ben-Yehuda
c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis.
EMBO Rep: 2011, 12(6);594-601
[PubMed:21566650] [WorldCat.org] [DOI] (I p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [WorldCat.org] [DOI] (P p)