YfhM

• Description: general stress protein, similar to epoxide hydrolase
  
  

Gene name	yfhM
Synonyms
Essential	no
Product	unknown
Function	survival of ethanol stress
Gene expression levels in SubtiExpress: yfhM
MW, pI	32 kDa, 6.064
Gene length, protein length	858 bp, 286 aa
Immediate neighbours	yfhL, csbB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

general stress proteins (controlled by SigB), cell envelope stress proteins (controlled by SigM, V, W, X, Y), poorly characterized/ putative enzymes, sporulation proteins

This gene is a member of the following regulons

SigB regulon, SigW regulon

The gene

Basic information

• Locus tag: BSU08590

Database entries

• BsubCyc: BSU08590

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU08590

• Structure:

• UniProt: O31581

• KEGG entry: [3]

• E.C. number:

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

• Operon: yfhK-yfhL-yfhM PubMed

• Expression browser: yfhM PubMed

• Sigma factor: SigB PubMed, SigW PubMed

• Regulation:
  • induced by stress (SigB) PubMed

• Regulatory mechanism:

• Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 194 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528] [WorldCat.org] [DOI] (P p)

H Antelmann, C Scharf, M Hecker
Phosphate starvation-inducible proteins of Bacillus subtilis: proteomics and transcriptional analysis.
J Bacteriol: 2000, 182(16);4478-90
[PubMed:10913081] [WorldCat.org] [DOI] (P p)

X Huang, A Gaballa, M Cao, J D Helmann
Identification of target promoters for the Bacillus subtilis extracytoplasmic function sigma factor, sigma W.
Mol Microbiol: 1999, 31(1);361-71
[PubMed:9987136] [WorldCat.org] [DOI] (P p)