ParC

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  • Description: subunit of DNA topoisomerase IV

Gene name parC
Synonyms grlA
Essential yes PubMed
Product subunit of DNA topoisomerase IV
Function chromosome segregation and compaction
Gene expression levels in SubtiExpress: parC
MW, pI 91 kDa, 6.307
Gene length, protein length 2418 bp, 806 aa
Immediate neighbours parE, ynfC
Sequences Protein DNA DNA_with_flanks
Genetic context
ParC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ParC expression.png


























Categories containing this gene/protein

DNA condensation/ segregation, essential genes

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU18100

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: topoisomerase gyrA/parC subunit family (according to Swiss-Prot)
  • Paralogous protein(s): GyrA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1WP5 (C-terminal domain, Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 67 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Serkalem Tadesse, Judita Mascarenhas, Bernd Kösters, Andrej Hasilik, Peter L Graumann
Genetic interaction of the SMC complex with topoisomerase IV in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 11);3729-3737
[PubMed:16272394] [WorldCat.org] [DOI] (P p)

Nora Au, Elke Kuester-Schoeck, Veena Mandava, Laura E Bothwell, Susan P Canny, Karen Chachu, Sierra A Colavito, Shakierah N Fuller, Eli S Groban, Laura A Hensley, Theresa C O'Brien, Amish Shah, Jessica T Tierney, Louise L Tomm, Thomas M O'Gara, Alexi I Goranov, Alan D Grossman, Charles M Lovett
Genetic composition of the Bacillus subtilis SOS system.
J Bacteriol: 2005, 187(22);7655-66
[PubMed:16267290] [WorldCat.org] [DOI] (P p)

Tung-Ju Hsieh, Lynn Farh, Wai Mun Huang, Nei-Li Chan
Structure of the topoisomerase IV C-terminal domain: a broken beta-propeller implies a role as geometry facilitator in catalysis.
J Biol Chem: 2004, 279(53);55587-93
[PubMed:15466871] [WorldCat.org] [DOI] (P p)

W M Huang, J L Libbey, P van der Hoeven, S X Yu
Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation.
Proc Natl Acad Sci U S A: 1998, 95(8);4652-7
[PubMed:9539793] [WorldCat.org] [DOI] (P p)