GabP

• Description: gamma-amino butyric acid permease and minor proline permease
  
  

Gene name	gabP
Synonyms	nrg-21
Essential	no
Product	gamma-amino butyric acid permease
Function	utilization of gamma-amino butyric acid
Gene expression levels in SubtiExpress: gabP
Metabolic function and regulation of this protein in SubtiPathways: gabP
MW, pI	50 kDa, 9.45
Gene length, protein length	1407 bp, 469 aa
Immediate neighbours	cotA, ydzX
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, utilization of amino acids, membrane proteins

This gene is a member of the following regulons

CodY regulon, TnrA regulon

The gene

Basic information

• Locus tag: BSU06310

Phenotypes of a mutant

• defective in the utilization of gamma-amino butyrate PubMed

Database entries

• BsubCyc: BSU06310

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • uptake of gamma-amino butyrate PubMed
  • minor transporter for proline (allows growth with proline in the absence of the major transporters PutP and OpuE) PubMed

• Protein family:
  • secondary amino acid transporter
  • amino acid, polyamine, organocation (APC) superfamily of transporters, TC 2.A.3
• Paralogous protein(s): AapA, HutM, RocC, RocE, YbgF, YbxG, YdgF, YtnA, YvbW

Extended information on the protein

• Kinetic information:
  • K(M) for gamma-amino butyrate: 77 +/- 17 µM PubMed
  • K(M) for proline: 700 +/- 330 µM PubMed

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU06310

• Structure:

• UniProt: P46349

• KEGG entry: [3]

• T.C. number: 2.A.3.1.5

Expression and regulation

• Operon: gabP PubMed

• Expression browser: gabP PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • TnrA: transcription activation PubMed
  • CodY: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 178 PubMed

Biological materials

• Mutant:
  • available in Erhard Bremer's lab PubMed

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Adrienne Zaprasis, Tamara Hoffmann, Lorena Stannek, Katrin Gunka, Fabian M Commichau, Erhard Bremer
The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.
J Bacteriol: 2014, 196(3);515-26
[PubMed:24142252] [WorldCat.org] [DOI] (I p)

C E Brechtel, S C King
4-Aminobutyrate (GABA) transporters from the amine-polyamine-choline superfamily: substrate specificity and ligand recognition profile of the 4-aminobutyrate permease from Bacillus subtilis.
Biochem J: 1998, 333 ( Pt 3)(Pt 3);565-71
[PubMed:9677314] [WorldCat.org] [DOI] (P p)

A E Ferson, L V Wray, S H Fisher
Expression of the Bacillus subtilis gabP gene is regulated independently in response to nitrogen and amino acid availability.
Mol Microbiol: 1996, 22(4);693-701
[PubMed:8951816] [WorldCat.org] [DOI] (P p)