DprA

• Description: conveys incoming ssDNA to RecA; facilitates the displacement of both SSBs (SsbB and SsbA), increases RecA nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing
  
  

Gene name	dprA
Synonyms	smf
Essential	no
Product	recombination mediator protein
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: dprA
Interactions involving this protein in SubtInteract: DprA
MW, pI	32 kDa, 9.755
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	sucD, topA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU16110

Phenotypes of a mutant

• • strongly impaired genetic recombination PubMed

Database entries

• BsubCyc: BSU16110

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds ssDNA, promotes displacement of SsbA and SsbB from ssDNA PubMed
  • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed

• Protein family: smf family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • forms dimers PubMed
  • DprA-RecA PubMed
  • DprA-DprA PubMed
  • DprA-ComFA PubMed
  • SsbB-DprA PubMed

• Localization:

Database entries

• BsubCyc: BSU16110

• Structure:

• UniProt: P39813

• KEGG entry: [3]

Expression and regulation

• Operon: dprA (according to DBTBS)

• Expression browser: dprA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger genetic competence (ComK) PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 57 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage