GntZ

• Description: 6-phosphogluconate dehydrogenase
  
  

Gene name	gntZ
Synonyms
Essential	no
Product	6-phosphogluconate dehydrogenase
Function	gluconate utilization
Gene expression levels in SubtiExpress: gntZ
Metabolic function and regulation of this protein in SubtiPathways: gntZ
MW, pI	51 kDa, 4.756
Gene length, protein length	1404 bp, 468 aa
Immediate neighbours	gntP, ahpC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GntR regulon

The gene

Basic information

• Locus tag: BSU40080

Database entries

• BsubCyc: BSU40080

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-D-gluconate + NADP⁺ = D-ribulose 5-phosphate + CO₂ + NADPH (according to Swiss-Prot)

• Protein family: 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s): GndA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU40080

• Structure:

• UniProt: P12013

• KEGG entry: [3]

• E.C. number: 1.1.1.44

Expression and regulation

• Operon:
  • gntR-gntK-gntP-gntZ PubMed1 PubMed2
  • gntZ PubMed

• Expression browser: gntZ PubMed

• Sigma factor:
  • gntR: SigA PubMed
  • gntZ: SigA PubMed

• Regulation:
  • induced by gluconate (GntR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GntR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 52 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1280 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Fujita, Y Miwa
Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein.
J Bacteriol: 1994, 176(2);511-3
[PubMed:8288545] [WorldCat.org] [DOI] (P p)

Y Miwa, Y Fujita
Promoter-independent catabolite repression of the Bacillus subtilis gnt operon.
J Biochem: 1993, 113(6);665-71
[PubMed:8370661] [WorldCat.org] [DOI] (P p)

A Reizer, J Deutscher, M H Saier, J Reizer
Analysis of the gluconate (gnt) operon of Bacillus subtilis.
Mol Microbiol: 1991, 5(5);1081-9
[PubMed:1659648] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita
The gluconate operon gnt of Bacillus subtilis encodes its own transcriptional negative regulator.
Proc Natl Acad Sci U S A: 1987, 84(13);4524-8
[PubMed:3037520] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa, J Nihashi, Y Aratani
Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
J Biol Chem: 1986, 261(29);13744-53
[PubMed:3020045] [WorldCat.org] (P p)

Y Fujita, J Nihashi, T Fujita
The characterization and cloning of a gluconate (gnt) operon of Bacillus subtilis.
J Gen Microbiol: 1986, 132(1);161-9
[PubMed:3011959] [WorldCat.org] [DOI] (P p)