CshB

• Description: DEAD-box RNA helicase, important for adaptation to low temperatures
  
  

Gene name	cshB
Synonyms	yqfR
Essential	no
Product	DEAD-box RNA helicase
Function	RNA helicase
Gene expression levels in SubtiExpress: cshB
Interactions involving this protein in SubtInteract: CshB
MW, pI	49 kDa, 9.89
Gene length, protein length	1314 bp, 438 aa
Immediate neighbours	nfo, yqfQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DEAD-box RNA helicases, translation

The gene

Basic information

• Locus tag: BSU25140

Phenotypes of a mutant

• poor growth at low temperatures (16 to 20°C) PubMed

Database entries

• BsubCyc: BSU25140

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • RNA helicase
  • required for ribosome assembly PubMed

• Protein family: DEAD-box RNA helicase

• Paralogous protein(s): CshA, DeaD, YfmL

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • CshB-CspB PubMed
  • CshB-DeaD PubMed

• Localization: cytoplasma, colocalizes with the ribosomes PubMed

Database entries

• BsubCyc: BSU25140

• Structure:

• UniProt: P54475

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: cshB PubMed

• Sigma factor:

• Regulation:
  • well expressed in complex medium, poorly expressed in minimal medium PubMed
  • expression declines in stationary phase PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 141 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 526 PubMed

Biological materials

• Mutant:
  • GP1051 (cat), available in Jörg Stülke's lab

• Expression vector:
  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1064 (spc), available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag construct:
  • GP1011 (spc, based on pGP1331), available in Jörg Stülke's lab

• Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

References

Additional publications: PubMed

Martin Lehnik-Habrink, Leonie Rempeters, Ákos T Kovács, Christoph Wrede, Claudia Baierlein, Heike Krebber, Oscar P Kuipers, Jörg Stülke
DEAD-Box RNA helicases in Bacillus subtilis have multiple functions and act independently from each other.
J Bacteriol: 2013, 195(3);534-44
[PubMed:23175651] [WorldCat.org] [DOI] (I p)

Martin Lehnik-Habrink, Henrike Pförtner, Leonie Rempeters, Nico Pietack, Christina Herzberg, Jörg Stülke
The RNA degradosome in Bacillus subtilis: identification of CshA as the major RNA helicase in the multiprotein complex.
Mol Microbiol: 2010, 77(4);958-71
[PubMed:20572937] [WorldCat.org] [DOI] (I p)

Karen Hunger, Carsten L Beckering, Frank Wiegeshoff, Peter L Graumann, Mohamed A Marahiel
Cold-induced putative DEAD box RNA helicases CshA and CshB are essential for cold adaptation and interact with cold shock protein B in Bacillus subtilis.
J Bacteriol: 2006, 188(1);240-8
[PubMed:16352840] [WorldCat.org] [DOI] (P p)

Carsten L Beckering, Leif Steil, Michael H W Weber, Uwe Völker, Mohamed A Marahiel
Genomewide transcriptional analysis of the cold shock response in Bacillus subtilis.
J Bacteriol: 2002, 184(22);6395-402
[PubMed:12399512] [WorldCat.org] [DOI] (P p)