ThiC

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  • Description: biosynthesis of the pyrimidine moiety of thiamine

Gene name thiC
Synonyms thiA
Essential no
Product unknown
Function biosynthesis of thiamine
Gene expression levels in SubtiExpress: thiC
Metabolic function and regulation of this protein in SubtiPathways:
thiC
MW, pI 65 kDa, 5.262
Gene length, protein length 1770 bp, 590 aa
Immediate neighbours ygaJ, ygaK
Sequences Protein DNA DNA_with_flanks
Genetic context
ThiC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ThiC expression.png




























Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

This gene is a member of the following regulons

Thi-box

The gene

Basic information

  • Locus tag: BSU08790

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: thiC family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-556 PubMed
    • phosphorylated on Ser-565, Ser-586 and Tyr-589 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]
  • E.C. number:

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Sigma factor:
  • Regulation:
    • repressed by thiamine and 2-methyl-4-amino-5-hydroxymethylpyrimidine PubMed
    • repressed by thiamine (Thi-box) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 10886 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 25260 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

T P Begley, D M Downs, S E Ealick, F W McLafferty, A P Van Loon, S Taylor, N Campobasso, H J Chiu, C Kinsland, J J Reddick, J Xi
Thiamin biosynthesis in prokaryotes.
Arch Microbiol: 1999, 171(5);293-300
[PubMed:10382260] [WorldCat.org] [DOI] (P p)


Original publications