YqeV

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  • Description: tRNA methylthiotransferase

Gene name yqeV
Synonyms mtaB
Essential no
Product tRNA methylthiotransferase
Function tRNA maturation
Gene expression levels in SubtiExpress: yqeV
Metabolic function and regulation of this protein in SubtiPathways:
YqeV
MW, pI 51 kDa, 5.242
Gene length, protein length 1353 bp, 451 aa
Immediate neighbours yqeW, yqeU
Sequences Protein DNA DNA_with_flanks
Genetic context
YqeV context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqeV expression.png















Categories containing this gene/protein

translation, heat shock proteins, membrane proteins

This gene is a member of the following regulons

HrcA regulon

The gene

Basic information

  • Locus tag: BSU25430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: modification of N(6)-threonylcarbamoyladenosine (t(6)A) to 2-methylthio-N(6)-threonylcarbamoyladenosine (ms(2)t(6)A) in tRNA PubMed
  • Protein family: YqeV subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YmcB

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): 2 (4Fe-4S) clusters PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Simon Arragain, Samuel K Handelman, Farhad Forouhar, Fan-Yan Wei, Kazuhito Tomizawa, John F Hunt, Thierry Douki, Marc Fontecave, Etienne Mulliez, Mohamed Atta
Identification of eukaryotic and prokaryotic methylthiotransferase for biosynthesis of 2-methylthio-N6-threonylcarbamoyladenosine in tRNA.
J Biol Chem: 2010, 285(37);28425-33
[PubMed:20584901] [WorldCat.org] [DOI] (I p)

Brian P Anton, Susan P Russell, Jason Vertrees, Simon Kasif, Elisabeth A Raleigh, Patrick A Limbach, Richard J Roberts
Functional characterization of the YmcB and YqeV tRNA methylthiotransferases of Bacillus subtilis.
Nucleic Acids Res: 2010, 38(18);6195-205
[PubMed:20472640] [WorldCat.org] [DOI] (I p)

G Homuth, A Mogk, W Schumann
Post-transcriptional regulation of the Bacillus subtilis dnaK operon.
Mol Microbiol: 1999, 32(6);1183-97
[PubMed:10383760] [WorldCat.org] [DOI] (P p)

G Homuth, S Masuda, A Mogk, Y Kobayashi, W Schumann
The dnaK operon of Bacillus subtilis is heptacistronic.
J Bacteriol: 1997, 179(4);1153-64
[PubMed:9023197] [WorldCat.org] [DOI] (P p)

G Yuan, S L Wong
Isolation and characterization of Bacillus subtilis groE regulatory mutants: evidence for orf39 in the dnaK operon as a repressor gene in regulating the expression of both groE and dnaK.
J Bacteriol: 1995, 177(22);6462-8
[PubMed:7592421] [WorldCat.org] [DOI] (P p)