TagA

• Description: UDP-N-acetyl-D-mannosamine transferase
  
  

Gene name	tagA
Synonyms
Essential	yes PubMed, no PubMed
Product	UDP-N-acetyl-D-mannosamine transferase
Function	biosynthesis of teichoic acid
Gene expression levels in SubtiExpress: tagA
MW, pI	29 kDa, 10.133
Gene length, protein length	768 bp, 256 aa
Immediate neighbours	tagD, tagB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall synthesis, biosynthesis of cell wall components

This gene is a member of the following regulons

PhoP regulon, WalR regulon

The gene

Basic information

• Locus tag: BSU35750

Phenotypes of a mutant

• essential PubMed, reduced growth rate, loss of rod-like morphology PubMed
• inactivation of tagA strongly restores beta-lactam resistance in a sigM mutant PubMed

Database entries

• BsubCyc: BSU35750

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: UDP-N-acetyl-D-mannosamine + N-acetyl-D-glucosaminyldiphosphoundecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenol (according to Swiss-Prot)

• Protein family: glycosyltransferase 26 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • secreted (according to Swiss-Prot)

Database entries

• BsubCyc: BSU35750

• Structure:

• UniProt: P27620

• KEGG entry: [3]

• E.C. number: 2.4.1.187

Expression and regulation

• Operon: tagA-tagB PubMed

• Expression browser: tagA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed when phosphate is available (PhoP) PubMed

• Regulatory mechanism:
  • PhoP: transcription repression PubMed
  • WalR: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Michael A D'Elia, James A Henderson, Terry J Beveridge, David E Heinrichs, Eric D Brown
The N-acetylmannosamine transferase catalyzes the first committed step of teichoic acid assembly in Bacillus subtilis and Staphylococcus aureus.
J Bacteriol: 2009, 191(12);4030-4
[PubMed:19376878] [WorldCat.org] [DOI] (I p)

Alex Formstone, Rut Carballido-López, Philippe Noirot, Jeffery Errington, Dirk-Jan Scheffers
Localization and interactions of teichoic acid synthetic enzymes in Bacillus subtilis.
J Bacteriol: 2008, 190(5);1812-21
[PubMed:18156271] [WorldCat.org] [DOI] (I p)

Alistair Howell, Sarah Dubrac, Kasper Krogh Andersen, David Noone, Juliette Fert, Tarek Msadek, Kevin Devine
Genes controlled by the essential YycG/YycF two-component system of Bacillus subtilis revealed through a novel hybrid regulator approach.
Mol Microbiol: 2003, 49(6);1639-55
[PubMed:12950927] [WorldCat.org] [DOI] (P p)

Y Qi, F M Hulett
Role of Pho-P in transcriptional regulation of genes involved in cell wall anionic polymer biosynthesis in Bacillus subtilis.
J Bacteriol: 1998, 180(15);4007-10
[PubMed:9683503] [WorldCat.org] [DOI] (P p)

W Liu, S Eder, F M Hulett
Analysis of Bacillus subtilis tagAB and tagDEF expression during phosphate starvation identifies a repressor role for PhoP-P.
J Bacteriol: 1998, 180(3);753-8
[PubMed:9457886] [WorldCat.org] [DOI] (P p)

C Mauël, A Bauduret, C Chervet, S Beggah, D Karamata
In Bacillus subtilis 168, teichoic acid of the cross-wall may be different from that of the cylinder: a hypothesis based on transcription analysis of tag genes.
Microbiology (Reading): 1995, 141 ( Pt 10);2379-89
[PubMed:7581998] [WorldCat.org] [DOI] (P p)

C Mauël, M Young, A Monsutti-Grecescu, S A Marriott, D Karamata
Analysis of Bacillus subtilis tag gene expression using transcriptional fusions.
Microbiology (Reading): 1994, 140 ( Pt 9);2279-88
[PubMed:7952180] [WorldCat.org] [DOI] (P p)