YqjM

• Description: NADPH-dependent flavin oxidoreductase
  
  

Gene name	yqjM
Synonyms
Essential	no
Product	NADPH-dependent flavin oxidoreductase
Function	reduction of double bonds of nonsaturated aldehydes and ketones, may be involved in detoxification
Gene expression levels in SubtiExpress: yqjM
MW, pI	37 kDa, 5.137
Gene length, protein length	1014 bp, 338 aa
Immediate neighbours	yqjN, yqjL
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

The gene

Basic information

• Locus tag: BSU23820

Database entries

• BsubCyc: BSU23820

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: NADPH + acceptor = NADP⁺ + reduced acceptor (according to Swiss-Prot)

• Protein family: NamA subfamily (according to Swiss-Prot)

• Paralogous protein(s): YqiG

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU23820

• Structure: 1Z41 (oxidized form), 1Z48 (reduced form)

• UniProt: P54550

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: yqjM (according to DBTBS)

• Expression browser: yqjM PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of H(2)O(2) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Karina Kitzing, Teresa B Fitzpatrick, Corinna Wilken, Justyna Sawa, Gleb P Bourenkov, Peter Macheroux, Tim Clausen
The 1.3 A crystal structure of the flavoprotein YqjM reveals a novel class of Old Yellow Enzymes.
J Biol Chem: 2005, 280(30);27904-13
[PubMed:15890652] [WorldCat.org] [DOI] (P p)

Teresa B Fitzpatrick, Sigrid Auweter, Karina Kitzing, Tim Clausen, Nikolaus Amrhein, Peter Macheroux
Structural and functional impairment of an Old Yellow Enzyme homologue upon affinity tag incorporation.
Protein Expr Purif: 2004, 36(2);280-91
[PubMed:15249051] [WorldCat.org] [DOI] (P p)

Teresa B Fitzpatrick, Nikolaus Amrhein, Peter Macheroux
Characterization of YqjM, an Old Yellow Enzyme homolog from Bacillus subtilis involved in the oxidative stress response.
J Biol Chem: 2003, 278(22);19891-7
[PubMed:12660247] [WorldCat.org] [DOI] (P p)