PdaC

• Description: peptidoglycan deacetylase C
  
  

Gene name	yjeA
Synonyms
Essential	no
Product	polysaccharide deacetylase C
Function	cell wall modification
MW, pI	53 kDa, 9.105
Gene length, protein length	1401 bp, 467 aa
Immediate neighbours	cotT, yjfA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall/ other

This gene is a member of the following regulons

WalR regulon

The gene

Basic information

• Locus tag: BSU12100

Phenotypes of a mutant

• sensitive to lysozyme treatment PubMed

Database entries

• BsubCyc: BSU12100

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • deacetylates N-acetylmuramic acid (MurNAc) but not GlcNAc from peptidoglycan PubMed

• Protein family: polysaccharide deacetylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:
  • K(m)=4.8 mM and k(cat)=0.32 s(-1) (toward B. subtilis peptidoglycan) PubMed
• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU12100

• Structure:

• UniProt: O34798

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation: repessed by WalR PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Letal I Salzberg, Leagh Powell, Karsten Hokamp, Eric Botella, David Noone, Kevin M Devine
The WalRK (YycFG) and σ(I) RsgI regulators cooperate to control CwlO and LytE expression in exponentially growing and stressed Bacillus subtilis cells.
Mol Microbiol: 2013, 87(1);180-95
[PubMed:23199363] [WorldCat.org] [DOI] (I p)

Kaori Kobayashi, I Putu Sudiarta, Takeko Kodama, Tatsuya Fukushima, Katsutoshi Ara, Katsuya Ozaki, Junichi Sekiguchi
Identification and characterization of a novel polysaccharide deacetylase C (PdaC) from Bacillus subtilis.
J Biol Chem: 2012, 287(13);9765-9776
[PubMed:22277649] [WorldCat.org] [DOI] (I p)

Ka-Lun Ng, Chui-Chi Lam, Zhibiao Fu, Yi-Fan Han, Karl W K Tsim, Wan-Keung R Wong
Cloning and characterization of the yjeA gene, encoding a novel deoxyribonuclease, from Bacillus subtilis.
J Biochem: 2007, 142(5);647-54
[PubMed:17878218] [WorldCat.org] [DOI] (P p)

Paola Bisicchia, David Noone, Efthimia Lioliou, Alistair Howell, Sarah Quigley, Thomas Jensen, Hanne Jarmer, Kevin M Devine
The essential YycFG two-component system controls cell wall metabolism in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);180-200
[PubMed:17581128] [WorldCat.org] [DOI] (P p)