SplB

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  • Description: spore photoproduct lyase

Gene name splB
Synonyms spl
Essential no
Product spore photoproduct lyase
Function protection of spore DNA against photodamage
Gene expression levels in SubtiExpress: splB
MW, pI 39 kDa, 8.524
Gene length, protein length 1026 bp, 342 aa
Immediate neighbours splA, ykwB
Sequences Protein DNA DNA_with_flanks
Genetic context
SplB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SplB expression.png















Categories containing this gene/protein

DNA repair/ recombination, sporulation proteins

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

  • Locus tag: BSU13930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • repairs a special thymine dimer (5-thyminyl-5,6-dihydrothymine), which is commonly called spore photoproduct at the early germination phase
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
    • contains a [4Fe-4S] cluster, uses the [4Fe-4S] cluster to reduce SAM
  • Effectors of protein activity:

Database entries

  • Structure: 4FHC (SplB from Geobacillus thermodenitrificans, 72% identity, 90% similarity) PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation in the forespore (SigG) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Alhosna Benjdia
DNA photolyases and SP lyase: structure and mechanism of light-dependent and independent DNA lyases.
Curr Opin Struct Biol: 2012, 22(6);711-20
[PubMed:23164663] [WorldCat.org] [DOI] (I p)

Structure of the spore photoproduct lesion in DNA

Isha Singh, Yajun Jian, Lei Li, Millie M Georgiadis
The structure of an authentic spore photoproduct lesion in DNA suggests a basis for recognition.
Acta Crystallogr D Biol Crystallogr: 2014, 70(Pt 3);752-9
[PubMed:24598744] [WorldCat.org] [DOI] (I p)

Original publications

Linlin Yang, Lei Li
The enzyme-mediated direct reversal of a dithymine photoproduct in germinating endospores.
Int J Mol Sci: 2013, 14(7);13137-53
[PubMed:23799365] [WorldCat.org] [DOI] (P e)

Linlin Yang, Renae S Nelson, Alhosna Benjdia, Gengjie Lin, Joshua Telser, Stefan Stoll, Ilme Schlichting, Lei Li
A radical transfer pathway in spore photoproduct lyase.
Biochemistry: 2013, 52(18);3041-50
[PubMed:23607538] [WorldCat.org] [DOI] (I p)

Alhosna Benjdia, Korbinian Heil, Thomas R M Barends, Thomas Carell, Ilme Schlichting
Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme.
Nucleic Acids Res: 2012, 40(18);9308-18
[PubMed:22761404] [WorldCat.org] [DOI] (I p)

Lei Li
Mechanistic studies of the radical SAM enzyme spore photoproduct lyase (SPL).
Biochim Biophys Acta: 2012, 1824(11);1264-77
[PubMed:22197590] [WorldCat.org] [DOI] (P p)

Linlin Yang, Gengjie Lin, Degang Liu, Karl J Dria, Joshua Telser, Lei Li
Probing the reaction mechanism of spore photoproduct lyase (SPL) via diastereoselectively labeled dinucleotide SP TpT substrates.
J Am Chem Soc: 2011, 133(27);10434-47
[PubMed:21671623] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

M Pedraza-Reyes, F Gutiérrez-Corona, W L Nicholson
Temporal regulation and forespore-specific expression of the spore photoproduct lyase gene by sigma-G RNA polymerase during Bacillus subtilis sporulation.
J Bacteriol: 1994, 176(13);3983-91
[PubMed:8021181] [WorldCat.org] [DOI] (P p)