YloS

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  • Description: thiamine pyrophosphokinase

Gene name yloS
Synonyms thiN
Essential no
Product thiamine pyrophosphokinase
Function thiamine salvage
Gene expression levels in SubtiExpress: yloS
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 23 kDa, 5.216
Gene length, protein length 642 bp, 214 aa
Immediate neighbours rpe, spoVM
Sequences Protein DNA DNA_with_flanks
Genetic context
YloS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YloS expression.png















Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU15800

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + thiamine = AMP + thiamine diphosphate (according to Swiss-Prot)
  • Protein family: thiamine pyrophosphokinase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications

Ghislain Schyns, Sébastien Potot, Yi Geng, Teresa M Barbosa, Adriano Henriques, John B Perkins
Isolation and characterization of new thiamine-deregulated mutants of Bacillus subtilis.
J Bacteriol: 2005, 187(23);8127-36
[PubMed:16291685] [WorldCat.org] [DOI] (P p)

Jonathan Melnick, Ewa Lis, Joo-Heon Park, Cynthia Kinsland, Hirotada Mori, Tomoya Baba, John Perkins, Ghislain Schyns, Olga Vassieva, Andrei Osterman, Tadhg P Begley
Identification of the two missing bacterial genes involved in thiamine salvage: thiamine pyrophosphokinase and thiamine kinase.
J Bacteriol: 2004, 186(11);3660-2
[PubMed:15150256] [WorldCat.org] [DOI] (P p)