ThiI

• Description: sulfuryl transferase, biosynthesis of 4-thiouridine in tRNA
  
  

Gene name	thiI
Synonyms	ytbJ
Essential	no
Product	sulfuryl transferase
Function	biosynthesis of 4-thiouridine in tRNA
Interactions involving this protein in SubtInteract: ThiI
MW, pI	40 kDa, 9.663
Gene length, protein length	1074 bp, 358 aa
Immediate neighbours	sspA, nifZ
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation

The gene

Basic information

• Locus tag: BSU29580

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: receives a sulfuryl group from NifZ and transfers it to uridine at position 8 of tRNA PubMed

• Protein family: thiI family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • NifZ-ThiI PubMed

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O34595

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ytbJ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lauren J Rajakovich, John Tomlinson, Patricia C Dos Santos
Functional Analysis of Bacillus subtilis Genes Involved in the Biosynthesis of 4-Thiouridine in tRNA.
J Bacteriol: 2012, 194(18);4933-40
[PubMed:22773787] [WorldCat.org] [DOI] (I p)

Joo-Heon Park, Pieter C Dorrestein, Huili Zhai, Cynthia Kinsland, Fred W McLafferty, Tadhg P Begley
Biosynthesis of the thiazole moiety of thiamin pyrophosphate (vitamin B1).
Biochemistry: 2003, 42(42);12430-8
[PubMed:14567704] [WorldCat.org] [DOI] (P p)