Gdh

• Description: glucose 1-dehydrogenase (NAD)
  
  

Gene name	gdh
Synonyms
Essential	no
Product	glucose 1-dehydrogenase (NAD)
Function	germination
Gene expression levels in SubtiExpress: gdh
MW, pI	27 kDa, 5.082
Gene length, protein length	783 bp, 261 aa
Immediate neighbours	glcU, ycnI
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

The gene

Basic information

• Locus tag: BSU03930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Beta-D-glucose + NAD(P)⁺ = D-glucono-1,5-lactone + NAD(P)H (according to Swiss-Prot)

• Protein family: short-chain dehydrogenases/reductases (SDR) family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P12310

• KEGG entry: [3]

• E.C. number: 1.1.1.47

Expression and regulation

• Operon: glcU-gdh PubMed

• Expression browser: gdh PubMed

• Sigma factor: SigG PubMed

• Regulation:
  • expressed during sporulation (SigG, SpoVT) PubMed

• Regulatory mechanism:
  • SpoVT: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

Y Nakatani, W L Nicholson, K D Neitzke, P Setlow, E Freese
Sigma-G RNA polymerase controls forespore-specific expression of the glucose dehydrogenase operon in Bacillus subtilis.
Nucleic Acids Res: 1989, 17(3);999-1017
[PubMed:2493633] [WorldCat.org] [DOI] (P p)

P N Rather, C P Moran
Compartment-specific transcription in Bacillus subtilis: identification of the promoter for gdh.
J Bacteriol: 1988, 170(11);5086-92
[PubMed:3141376] [WorldCat.org] [DOI] (P p)

Y Fujita, R Ramaley, E Freese
Location and properties of glucose dehydrogenase in sporulating cells and spores of Bacillus subtilis.
J Bacteriol: 1977, 132(1);282-93
[PubMed:21162] [WorldCat.org] [DOI] (P p)