FolK

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  • Description: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase

Gene name folK
Synonyms
Essential no
Product 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase
Function biosynthesis of folate
Gene expression levels in SubtiExpress: folK
Metabolic function and regulation of this protein in SubtiPathways:
Folate
MW, pI 18 kDa, 4.531
Gene length, protein length 501 bp, 167 aa
Immediate neighbours folB, yazB
Sequences Protein DNA Advanced_DNA
Genetic context
FolK context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FolK expression.png




























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00790

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine = AMP + (2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate (according to Swiss-Prot)
  • Protein family: HPPK family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1IM6 (from Escherichia coli, 47% identity, 61% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Antoine de Saizieu, Pierre Vankan, Cassandra Vockler, Adolphus P G M van Loon
The trp RNA-binding attenuation protein (TRAP) regulates the steady-state levels of transcripts of the Bacillus subtilis folate operon.
Microbiology (Reading): 1997, 143 ( Pt 3);979-989
[PubMed:9084182] [WorldCat.org] [DOI] (P p)